Na+ gradient-coupled porters of EScherichia coli share a common subunit.

Immunogenicity of a Fusion Protein Comprising Coli Surface Antigen 3 and Labile B Subunit of Enterotoxigenic Escherichia coli

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

production of pentameric cholera toxin b subunit in escherichia coli

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

Subunit dynamics in Escherichia coli preprotein translocase.

SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase. To measure the in vivo association of these polypeptides and assay possible exchange, plasmid-borne secY and secE genes were placed under control of the ara regulon and fused to DNA encoding the influenza hemagglutinin epitope. Cells were incubated with [35S]methionine, grown for a...

immunogenicity of a fusion protein comprising coli surface antigen 3 and labile b subunit of enterotoxigenic escherichia coli

background: enterotoxigenic escherichia coli (etec) strains are the major causes of diarrheal disease in humans and animals. colonization factors and enterotoxins are the major virulence factors in etec pathogenesis. for the broad-spectrum protection against etec, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. methods: a fusion protein is composed of a...

Coupled nucleoside phosphorylase reactions in Escherichia coli.